Journal
STEM CELLS INTERNATIONAL
Volume 2022, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2022/3222427
Keywords
-
Categories
Funding
- National Natural Science Foundation of China
- Natural Science Foundation of Guangdong Province
- Natural Science Foundation of Shenzhen City
- District Key Laboratory Fund of Longgang District of Shenzhen City
- Foundation of Beijing University of Chinese Medicine Department of Hepatopathy, Shenzhen Hospital
- [82172107]
- [2020A1515011314]
- [2021A1515011927]
- [2020B121206003]
- [JCYJ20190807101401682]
- [JCYJ20210324135014040]
- [LGKCZSYS2019000051]
- [2020-BUCMSZYLRC04]
Ask authors/readers for more resources
By including BMP4 in the hepatic differentiation medium, we have achieved co-differentiation of endoderm-derived hepatocytes and mesoderm-derived nonparenchymal cells, which has a beneficial effect on hepatocyte maturation and lifespan in vitro.
To date, hepatocytes derived from human-induced pluripotent stem cells (hiPSC) provide a potentially unlimited resource for clinical application and drug development. However, most hiPSC-derived hepatocyte-like cells initiated differentiation from highly purified definitive endoderm, which are insufficient to accurately replicate the complex regulation of signals among multiple cells and tissues during liver organogenesis, thereby displaying an immature phenotypic and short survival time in vitro. Here, we described a protocol to achieve codifferentiation of endoderm-derived hepatocytes and mesoderm-derived nonparenchymal cells by the inclusion of BMP4 into hepatic differentiation medium, which has a beneficial effect on the hepatocyte maturation and lifespan in vitro. Our codifferentiation system suggests the important role of nonparenchymal cells in liver organogenesis. Hopefully, these hepatocytes described here provide a promising approach in the therapy of liver diseases.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available