Journal
NUCLEUS
Volume 14, Issue 1, Pages -Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/19491034.2023.2165602
Keywords
PRR14; heterochromatin; nuclear lamina; HP1; FRAP
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This study demonstrates that Proline Rich 14 (PRR14) protein organizes H3K9me3-modified heterochromatin at the nuclear lamina. It is shown that PRR14 associates with both the nuclear lamina and heterochromatin, and can reorganize heterochromatin in interphase cells independently of mitosis. The study proposes a model of dynamic heterochromatin organization at the nuclear lamina via the PRR14 tethering protein.
The eukaryotic genome is organized in three dimensions within the nucleus. Transcriptionally active chromatin is spatially separated from silent heterochromatin, a large fraction of which is located at the nuclear periphery. However, the mechanisms by which chromatin is localized at the nuclear periphery remain poorly understood. Here we demonstrate that Proline Rich 14 (PRR14) protein organizes H3K9me3-modified heterochromatin at the nuclear lamina. We show that PRR14 dynamically associates with both the nuclear lamina and heterochromatin, and is able to reorganize heterochromatin in the nucleus of interphase cells independent of mitosis. We characterize two functional HP1-binding sites within PRR14 that contribute to its association with heterochromatin. We also demonstrate that PPR14 forms an anchoring surface for heterochromatin at the nuclear lamina where it interacts dynamically with HP1-associated chromatin. Our study proposes a model of dynamic heterochromatin organization at the nuclear lamina via the PRR14 tethering protein.
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