Journal
MICROMACHINES
Volume 13, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/mi13122107
Keywords
screen-printed microchip; molecularly imprinted polymer; drug detection; metronidazole benzoate
Categories
Funding
- Taif University Researcher Support , Taif University, Taif, Saudi Arabia
- [TURSP-2020/30]
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This study fabricated a novel potentiometric microelectrode incorporating a molecularly imprinted polymer (MIP), and successfully utilized it for the recognition and quantification of metronidazole benzoate. The MIP-based sensor exhibited a super-Nernstian response, fast response time, and high selectivity, enabling accurate and precise quantification of metronidazole benzoate in real samples.
A novel potentiometric microelectrode incorporating a molecularly imprinted polymer (MIP) was fabricated, characterized, and successfully applied to the recognition and quantification of the drug, metronidazole benzoate. The elaborated MIP-based sensor was realized by thermal polarization, using metronidazole benzoate as the template material, 1-vinyl-2-pyrrolidine (VP) as a functional monomer, and ethylene glycol dimethacrylate (EGDMA) as the cross-linking agent in the presence of benzoyl peroxide as the initiator. The MIP-based sensor exhibited a super-Nernstian response (61.5 +/- 0.5, mV/decade) covering the linear concentration range of 1 x 10(-8)-1 x 10(-3) mole L-1 of metronidazole benzoate with a fast response time (<= 10, s.) and detection limit of 7 x 10(-9) mole L-1. The microchip showed high selectivity toward the template drug molecule in the presence of many investigated interfering species. The chip electrode was successfully used in the quantification of metronidazole benzoate in some real biological samples with high accuracy (recovery, 95.4%) and precision (RSD, 1.5). Moreover, the merits offered by the elaborated MIP-based MB microchip assembly include small size, miniaturization, integration, and consequently, automation feasibility.
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