Forage quality and physiological performance of mowed alfalfa (Medicago sativa L.) subjected to combined light quality and drought

Alfalfa (Medicago sativa) can dwell in water-deficient habitats, where it is difficult to predict dry mass (DM) production and forage quality due to understory transmittance. Mowing is a recommended practice for alfalfa populations under drought, but its effect on forested land receives less attention. In a controlled indoor experiment, we found that drought better reduces shoot DM weight and crude fiber content (CFi) in blue light (33.7% red, 48.5% green, and 17.8% blue lights) than red light (71.7% red, 13.7% green, and 14.6% blue lights). Mowing decreases carbon (C) isotope signature (delta C-13), CFi, and total C content in shoots but increases their accumulations in DM, nonstructural carbohydrates, and crude fat content (CFa). The results also demonstrated that mown alfalfa has higher starch content when exposed to green light (26.2% red, 56.4% green, and 17.4% blue lights) compared to the other two spectra. Multiple factorial regression indicated that higher soluble sugar content accounted for the increase of CFa and DM weight for CFi. Overall, mowing in blue-light-enriched understory stands is recommended and produces high-forage-quality alfalfa, which can be used as a lowered crude fiber component.

Automated summary

This study found that drought has a greater impact on shoot dry mass and crude fiber content in alfalfa under blue light compared to red light. Mowing reduces carbon isotope signature, crude fiber content, and total carbon content in shoots but increases accumulations in dry mass, nonstructural carbohydrates, and crude fat content. Mown alfalfa exposed to green light has higher starch content.