4.6 Review

Application of transposon insertion site sequencing method in the exploration of gene function in microalgae

Related references

Note: Only part of the references are listed.
Review Cell Biology

Roles of mRNA poly(A) tails in regulation of eukaryotic gene expression

Lori A. Passmore et al.

Summary: Recent studies have shown that poly(A) tails play a complex role in the cytoplasm, including promoting translation and controlling mRNA stability through deadenylation. The length of poly(A) tails is correlated with translation rate and mRNA lifespan, indicating a significant role in gene regulation.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2022)

Article Environmental Sciences

Recovery of value-added products by mining microalgae

Ranbin Liu et al.

Summary: Microalgae blooms can have negative impacts on the aquatic environment, but they can also be used as resources to replace fossil fuels and plastics. In addition, microalgae can absorb nutrients from wastewater, making it a valuable approach to increase wastewater value. However, specific technical protocols need to be developed for different microalgae products and wastewater types.

JOURNAL OF ENVIRONMENTAL MANAGEMENT (2022)

Article Plant Sciences

Small antisense RNA ThfR positively regulates Thf1 in Synechocystis sp. PCC 6803

Xiang Li et al.

Summary: This study discovered a small antisense RNA gene (ThfR) that interacts with the thf1 gene, regulating photosynthesis under nitrogen starvation or high-light stress, and enhancing the growth ability of cyanobacteria under high-light stress.

JOURNAL OF PLANT PHYSIOLOGY (2022)

Article Genetics & Heredity

Systematic characterization of gene function in the photosynthetic alga Chlamydomonas reinhardtii

Friedrich Fauser et al.

Summary: This study utilized a barcoded mutant library to systematically analyze the phenotypes of Chlamydomonas reinhardtii mutants under different environmental conditions and chemical treatments. The results provide insights into the functions of numerous genes and can inform the understanding of gene function in land plants.

NATURE GENETICS (2022)

Review Biochemistry & Molecular Biology

Hydrogel-Forming Algae Polysaccharides: From Seaweed to Biomedical Applications

Marco Beaumont et al.

Summary: This review provides an overview of the journey of polysaccharides extracted from algae to biomedical applications, including algae culture, extraction, structure-properties relationships, chemical modifications, and characterization techniques. The focus is on algae polysaccharides that form hydrogels and their gelation mechanisms.

BIOMACROMOLECULES (2021)

Article Biology

Molecular characterization and expression of cyclic nucleotide gated ion channels 19 and 20 in Arabidopsis thaliana for their potential role in salt stress

Sadaf Oranab et al.

Summary: CNGC19 and CNGC20 play significant roles in salt stress response in A. thaliana, with increased expression levels after 6 hours of high salt stress. The T-DNA insertion sites for activation tagged mutants of CNGC19 and CNGC20 under salt stress were determined using TAIL-PCR technique.

SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2021)

Letter Medical Laboratory Technology

Development of an inverse-PCR approach for characterization of the major BCR-ABL1 breakpoint sequences on genomic DNA: proof of concept

Leandro G. Gutierrez et al.

CLINICAL CHEMISTRY AND LABORATORY MEDICINE (2021)

Article Food Science & Technology

The potentials and challenges of using microalgae as an ingredient to produce meat analogues

Yunlei Fu et al.

Summary: This article explores various ways to process microalgae into meat analogues, focusing on the nutritional potential of microalgae and the challenges in producing meat analogues. Microalgae contain rich protein and diverse nutraceutical compounds, enabling the food industry to produce meat analogues with high nutritional value. However, the current technological route for producing microalgal-based meat analogues is incomplete.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2021)

Article Chemistry, Analytical

Identification of the insertion site of transgenic DNA based on cyclization of the target gene with the flanking sequence and nested inverse PCR

Jiayu Wang et al.

Summary: This study presents a novel method for identifying the insertion site of transgenic DNA by constructing circular DNA, which demonstrated efficient ligation properties of T7 DNA ligase. The method showed high specificity and sensitivity, making it a practical tool for detecting transgenic DNA and could potentially be extended for gene doping detection and assessment of genetically modified organisms.

TALANTA OPEN (2021)

Article Biology

ATAC-Seq-based Identification of Extrachromosomal Circular DNA in Mammalian Cells and Its Validation Using Inverse PCR and FISH

Zhangli Su et al.

Summary: Recent studies have highlighted the importance of eccDNA from yeast to humans, particularly in cancer cells where oncogenes are amplified on eccDNA causing genomic instability. A new method independent of rolling circle amplification was developed by the research team, utilizing ATAC-seq and a Circle_finder algorithm to predict eccDNA, followed by validation through inverse PCR and FISH.

BIO-PROTOCOL (2021)

Review Biochemistry & Molecular Biology

Tn5 Transposase Applied in Genomics Research

Niannian Li et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

Review Food Science & Technology

Algae in food: a general review

Sylwia Scieszka et al.

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2019)

Letter Biochemistry & Molecular Biology

A modified high-efficiency thermal asymmetric interlaced PCR method for amplifying long unknown flanking sequences

Jiantao Tan et al.

JOURNAL OF GENETICS AND GENOMICS (2019)

Article Microbiology

Small Antisense RNA RbIR Positively Regulates RuBisCo in Synechocystis sp PCC 6803

Jinlu Hu et al.

FRONTIERS IN MICROBIOLOGY (2017)

Article Engineering, Environmental

Effective Biological DeNOx of Industrial Flue Gas by the Mixotrophic Cultivation of an Oil-Producing Green Alga Chlorella sp C2

Weixian Chen et al.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2016)

Article Multidisciplinary Sciences

Unique attributes of cyanobacterial metabolism revealed by improved genome-scale metabolic modeling and essential gene analysis

Jared T. Broddrick et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2016)

Article Multidisciplinary Sciences

The essential gene set of a photosynthetic organism

Benjamin E. Rubin et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)

Article Biotechnology & Applied Microbiology

Characterisation of defatted Scenedesmus dimorphus algal biomass as animal feed

S. Vidyashankar et al.

JOURNAL OF APPLIED PHYCOLOGY (2015)

Article Multidisciplinary Sciences

Linear Amplification Mediated PCR - Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Richard Gabriel et al.

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS (2014)

Article Biotechnology & Applied Microbiology

Use of secondary-treated wastewater for the production of Muriellopsis sp.

C. Gomez et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2013)

Article Agricultural Engineering

Comparing the use of different domestic wastewaters for coupling microalgal production and nutrient removal

Iago Teles Dominguez Cabanelas et al.

BIORESOURCE TECHNOLOGY (2013)

Review Microbiology

Transposon insertion sequencing: a new tool for systems-level analysis of microorganisms

Tim van Opijnen et al.

NATURE REVIEWS MICROBIOLOGY (2013)

Article Environmental Sciences

Microalgae: a promising tool for carbon sequestration

Uday Bhan Singh et al.

MITIGATION AND ADAPTATION STRATEGIES FOR GLOBAL CHANGE (2013)

Article Biochemical Research Methods

A new method to identify flanking sequence tags in chlamydomonas using 3′-RACE

Laurence Meslet-Cladiere et al.

PLANT METHODS (2012)

Article Biotechnology & Applied Microbiology

Global gene disruption in human cells to assign genes to phenotypes by deep sequencing

Jan E. Carette et al.

NATURE BIOTECHNOLOGY (2011)

Article Genetics & Heredity

A transposon toolkit for gene transfer and mutagenesis in protozoan parasites

Jeziel D. Damasceno et al.

GENETICA (2010)

Review Genetics & Heredity

Mobile DNA and evolution in the 21st century

James A. Shapiro

MOBILE DNA (2010)

Review Biotechnology & Applied Microbiology

Applications of Transposon-Based Gene Delivery System in Bacteria

Kyoung-Hee Choi et al.

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY (2009)

Article Biochemical Research Methods

Using an Inverse PCR Method to Clone the Wheat Cytokinin Oxidase/Dehydrogenase Gene TaCKX1

De-Shun Feng et al.

PLANT MOLECULAR BIOLOGY REPORTER (2008)

Article Biotechnology & Applied Microbiology

T-DNA insertion, plasmid rescue and integration analysis in the model mycorrhizal fungus Laccaria bicolor

Minna Kemppainen et al.

MICROBIAL BIOTECHNOLOGY (2008)

Article Biochemical Research Methods

High-resolution insertion-site analysis by linear amplification-mediated PCR (LAM-PCR)

Schmidt Manfred et al.

NATURE METHODS (2007)

Article Biochemical Research Methods

High-efficiency thermal asymmetric interlaced PCR for amplification of unknown flanking sequences

Yao-Guang Liu et al.

BIOTECHNIQUES (2007)

Article Multidisciplinary Sciences

Gene expression analysis of plant host-pathogen interactions by SuperSAGE

H Matsumura et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2003)