Detection and homology analysis of carbapenem resistant Acinetobacter baumannii resistance gene

ObjectiveTo explore the carrying status and homology of carbapenem resistant Acinetobacter baumannii (CRAB) in our hospital. MethodsFrom January 2015 to December 2017, 52 strains of acinetobacter baumannii isolated from the bacteria room of the clinical laboratory of Baogang hospital in Inner Mongolia were selected as the research object. K-B disk diffusion method and Vitek-2 were used to determine the drug sensitivity of Acinetobacter baumannii. The drug resistance gene was detected by polymerase chain reaction (PCR) and its homology was analyzed by pulsed field gel electrophoresis (PFGE). ResultsExcept for Cefoperazone/sulbactam, other antibiotics were resistant to ab. The detection rate of drug resistance gene class C beta-lactamases (ADC) was 100%, and the higher detection rates of other drug resistance genes were class D beta-lactamases (OXA)-51 (36 strains, 90.0%),disinfectant gene qacEo1-sull (32 strains, 80.0%), and klebsiella pneumoniae carbapenemase (KPC) gene was not detected. 2-8 drug resistance genes were detected in each CRAB strain, and the strains with 6 drug resistance genes were the most (15 strains, 37.5%); Among the detected drug-resistant gene combinations, ADC+OXA-23 + OXA-51 gene was detected at the same time (29 strains, 72.5%), followed by ADC+ intl1 + qacE o 1-sull gene (26 strains, 65.0%), ADC + qacE o 1-sull + ant (3 '') -i gene (19 strains, 47.5%), and 11 strains (27.5%). There were 19 different types in PFGE homology test, each type was 1-9 strains, including 9 strains of A5 type and 8 strains of A18 type, mainly from intensive care unit. ConclusionCRAB in the hospital is highly resistant to common clinical antibiotics. OXA-23 and OXA-51 genes are most likely to be the main factors causing drug resistance of Acinetobacter baumannii in the hospital. Homology analysis showed that there was CRAB nosocomial infection transmission in different wards of the hospital.

Automated summary

The study aimed to investigate the carrying status and homology of carbapenem resistant Acinetobacter baumannii (CRAB) in our hospital. A total of 52 strains of Acinetobacter baumannii isolated from the bacteria room of the clinical laboratory of Baogang hospital in Inner Mongolia from January 2015 to December 2017 were selected as the research object. Drug sensitivity, drug resistance gene detection, and homology analysis were conducted. The results showed high resistance of CRAB in the hospital, with OXA-23 and OXA-51 genes being the main factors causing drug resistance.