4.8 Article

Initiation of HIV-1 Gag lattice assembly is required for recognition of the viral genome packaging signal

Related references

Note: Only part of the references are listed.
Article Virology

HIV-1 Packaging Visualised by In-Gel SHAPE

Aaron R. D'Souza et al.

Summary: HIV-1 packages two copies of its gRNA into virions through an interaction with the Gag protein, essential for virion infectivity. A lack of structural techniques previously hindered the understanding of the stepwise packaging process. The study using in-gel SHAPE technique sheds light on the initiation of HIV-1 packaging and the interaction between RNA and Gag protein.

VIRUSES-BASEL (2021)

Article Microbiology

Plasma Membrane Anchoring and Gag:Gag Multimerization on Viral RNA Are Critical Properties of HIV-1 Gag Required To Mediate Efficient Genome Packaging

Alice Duchon et al.

Summary: HIV-1 Gag selects and packages the HIV RNA genome during virus assembly, with multimerization and plasma membrane anchoring properties playing critical roles in efficient genome packaging. Studying these properties can provide insights into the mechanisms of viral RNA packaging and assembly of infectious virions.
Article Multidisciplinary Sciences

A stable immature lattice packages IP6 for HIV capsid maturation

Donna L. Mallery et al.

Summary: HIV maintains immature lattice stability to ensure IP6 packaging while allowing maturation. Mutant viruses with reduced IP6 recruitment show increased infectivity upon treatment with MIs or second-site compensatory mutations.

SCIENCE ADVANCES (2021)

Article Microbiology

HIV-1 matrix-tRNA complex structure reveals basis for host control of Gag localization

Charles Bou-Nader et al.

Summary: The HIV-1 virion structural polyprotein Gag is directed to assembly sites by its N-terminal matrix domain, which also binds to host tRNAs. The co-crystal structure of the HIV-1 MA-tRNA(Lys3) complex reveals a specialized interface that facilitates binding in solution and living cells. Mutations disrupting this interaction cause Gag redistribution and reduced HIV-1 replication, indicating the virus's reliance on host tRNAs for subcellular localization control.

CELL HOST & MICROBE (2021)

Article Multidisciplinary Sciences

5′-Cap sequestration is an essential determinant of HIV-1 genome packaging

Pengfei Ding et al.

Summary: The study reveals a bipartite mechanism for RNA packaging in HIV-1 assembly, involving both sequestration of the 5' cap and exposure of NC binding sites in the dimeric leader region. Certain elements and structures within the leader are crucial for efficient and accurate packaging.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

Article Virology

Derivation and characterization of an HIV-1 mutant that rescues IP6 binding deficiency

Daniel Poston et al.

Summary: The assembly and maturation of HIV-1 virions rely on cellular polyanion IP6 binding to primary amines on Gag or CA hexamers. Mutations affecting IP6 binding can lead to assembly deficits, but compensatory mutations like T371I can restore replication competence. Moreover, the maturation inhibitor Bevirimat (BVM) can also rescue assembly and replication of IP6-binding deficient mutants, offering insights into HIV-1 virion assembly events.

RETROVIROLOGY (2021)

Article Biochemical Research Methods

Clip for studying protein-RNA interactions that regulate virus replication

Christian Shema Mugisha et al.

METHODS (2020)

Article Multidisciplinary Sciences

Identification of the initial nucleocapsid recognition element in the HIV-1 RNA packaging signal

Pengfei Ding et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

Article Multidisciplinary Sciences

Modeling the dynamics and kinetics of HIV-1 Gag during viral assembly

Michael D. Tomasini et al.

PLOS ONE (2018)

Article Multidisciplinary Sciences

Inositol phosphates are assembly co-factors for HIV-1

Robert A. Dick et al.

NATURE (2018)

Article Biochemical Research Methods

irCLIP platform for efficient characterization of protein-RNA interactions

Brian J. Zarnegar et al.

NATURE METHODS (2016)

Article Multidisciplinary Sciences

An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation

Florian K. M. Schur et al.

SCIENCE (2016)

Article Biology

Crystal structure of an HIV assembly and maturation switch

Jonathan M. Wagner et al.

ELIFE (2016)

Article Multidisciplinary Sciences

Structure of the HIV-1 RNA packaging signal

Sarah C. Keane et al.

SCIENCE (2015)

Article Biochemistry & Molecular Biology

Global Changes in the RNA Binding Specificity of HIV-1 Gag Regulate Virion Genesis

Sebla B. Kutluay et al.

Article Multidisciplinary Sciences

Computational design of water-soluble α-helical barrels

Andrew R. Thomson et al.

SCIENCE (2014)

Article Biochemistry & Molecular Biology

A Trimer of Dimers Is the Basic Building Block for Human Immunodeficiency Virus-1 Capsid Assembly

Manuel Tsiang et al.

BIOCHEMISTRY (2012)

Article Biophysics

Early Stages of the HIV-1 Capsid Protein Lattice Formation

John M. A. Grime et al.

BIOPHYSICAL JOURNAL (2012)

Article Virology

Functional Redundancy in HIV-1 Viral Particle Assembly

Ina P. O'Carroll et al.

JOURNAL OF VIROLOGY (2012)

Article Virology

On the Role of the SP1 Domain in HIV-1 Particle Assembly: a Molecular Switch?

Siddhartha A. K. Datta et al.

JOURNAL OF VIROLOGY (2011)

Article Microbiology

Analysis of the Initiating Events in HIV-1 Particle Assembly and Genome Packaging

Sebla B. Kutluay et al.

PLOS PATHOGENS (2010)

Article Biochemical Research Methods

The Sequence Alignment/Map format and SAMtools

Heng Li et al.

BIOINFORMATICS (2009)

Article Biochemistry & Molecular Biology

X-Ray Structures of the Hexameric Building Block of the HIV Capsid

Owen Pornillos et al.

Article Multidisciplinary Sciences

Imaging the interaction of HIV-1 genomes and Gag during assembly of individual viral particles

Nolwenn Jouvenet et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2009)

Article Biotechnology & Applied Microbiology

Ultrafast and memory-efficient alignment of short DNA sequences to the human genome

Ben Langmead et al.

GENOME BIOLOGY (2009)

Article Virology

Selective and nonselective packaging of cellular RNAs in retrovirus particles

Samuel J. Rulli et al.

JOURNAL OF VIROLOGY (2007)

Article Multidisciplinary Sciences

A seven-helix coiled coil

Jie Liu et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2006)

Review Microbiology

How retroviruses select their genomes

V D'Souza et al.

NATURE REVIEWS MICROBIOLOGY (2005)