Journal
CELL CYCLE
Volume 14, Issue 15, Pages 2394-2398Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15384101.2015.1053673
Keywords
Cell cycle; Mitosis; Embryo; C. elegans
Categories
Funding
- PhD fellowship from Fondation ARC pour la Recherche sur le Cancer, the City of Paris
- Who am I? Laboratory of Excellence [ANR-11-LABX-0071]
- French Government through Investments for the Future program [ANR-11-IDEX-0005-01]
- Swiss National Science Foundation [31003A_156013]
- University of Geneva
- French National Research Agency [ANR-2012-BSV2-0001-01]
- Foundation for Medical Research Equipe FRM [DEQ20140329538]
- Swiss National Science Foundation (SNF) [31003A_156013] Funding Source: Swiss National Science Foundation (SNF)
Ask authors/readers for more resources
Mitosis is orchestrated by several protein kinases including Cdks, Plks and Aurora kinases. Despite considerable progress toward understanding the individual function of these protein kinases, how their activity is coordinated in space and time during mitosis is less well understood. In a recent article published in the Journal of Cell Biology, we show that CDK-1 regulates PLK-1 activity during mitosis in C. elegans embryos through multisite phosphorylation of the PLK-1 activator SPAT-1 (Aurora Borealis, Bora in human). SPAT-1 variants mutated on CDK-1 phosphorylation sites results in severe delays in mitotic entry, mimicking embryos lacking spat-1 or plk-1 function. We further show that SPAT-1 phosphorylation by CDK-1 promotes its binding to PLK-1 and stimulates PLK-1 phosphorylation on its activator T-loop by Aurora A kinase in vitro. Likewise, we find that phosphorylation of Bora by Cdk1 promotes phosphorylation of human Plk1 by Aurora A suggesting that this mechanism is conserved in humans. These results indicate that Cdk1 regulates Plk1 by boosting its kinase activity. Here we discuss these recent findings and open questions regarding the regulation of Plk1/PLK-1 by Cdk1/CDK-1 and Bora/SPAT-1.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available