4.7 Article

Muscle-like Scaffolds for Biomechanical Stimulation in a Custom-Built Bioreactor

Journal

POLYMERS
Volume 14, Issue 24, Pages -

Publisher

MDPI
DOI: 10.3390/polym14245427

Keywords

tissue engineering; myoblasts; viability; cell adhesion; bioreactor

Funding

  1. Technological Institute of Costa Rica Research Vice Rectory
  2. Postgraduate studies office of the Technological Institute of Costa Rica [1490005]
  3. [25-2018]

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Tissue engineering aims to develop in-vitro substitutes of native tissues, and one approach is using bioreactors combined with biomimetic scaffolds. This study demonstrated that a collagen-coated microfilament scaffold seeded with C2C12 myoblasts inside a custom-built bioreactor provided a promising structure for the study of skeletal muscle models. The findings have important implications for mechanical stimulation studies in tissue engineering applications.
Tissue engineering aims to develop in-vitro substitutes of native tissues. One approach of tissue engineering relies on using bioreactors combined with biomimetic scaffolds to produce study models or in-vitro substitutes. Bioreactors provide control over environmental parameters, place and hold a scaffold under desired characteristics, and apply mechanical stimulation to scaffolds. Polymers are often used for fabricating tissue-engineering scaffolds. In this study, polycaprolactone (PCL) collagen-coated microfilament scaffolds were cell-seeded with C2C12 myoblasts; then, these were grown inside a custom-built bioreactor. Cell attachment and proliferation on the scaffolds were investigated. A loading pattern was used for mechanical stimulation of the cell-seeded scaffolds. Results showed that the microfilaments provided a suitable scaffold for myoblast anchorage and that the custom-built bioreactor provided a qualified environment for the survival of the myoblasts on the polymeric scaffold. This PCL-based microfilament scaffold located inside the bioreactor proved to be a promising structure for the study of skeletal muscle models and can be used for mechanical stimulation studies in tissue engineering applications.

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