The Prmt5-Vasa module is essential for spermatogenesis in Bombyx mori

In lepidopteran insects, dichotomous spermatogenesis produces eupyrene spermatozoa, which are nucleated, and apyrene spermatozoa, which are anucleated. Both sperm morphs are essential for fertilization, as eupyrene sperm fertilize the egg, and apyrene sperm is necessary for the migration of eupyrene sperm. In Drosophila, Prmt5 acts as a type II arginine methyltransferase that catalyzes the symmetrical dimethylation of arginine residues in the RNA helicase Vasa. Prmt5 is critical for the regulation of spermatogenesis, but Vasa is not. To date, functional genetic studies of spermatogenesis in the lepidopteran model Bombyx mori has been limited. In this study, we engineered mutations in BmPrmt5 and BmVasa through CRISPR/Cas9-based gene editing. Both BmPrmt5 and BmVasa loss-of-function mutants had similar male and female sterility phenotypes. Through immunofluorescence staining analysis, we found that the morphs of sperm from both BmPrmt5 and BmVasa mutants have severe defects, indicating essential roles for both BmPrmt5 and BmVasa in the regulation of spermatogenesis. Mass spectrometry results identified that R35, R54, and R56 of BmVasa were dimethylated in WT while unmethylated in BmPrmt5 mutants. RNA-seq analyses indicate that the defects in spermatogenesis in mutants resulted from reduced expression of the spermatogenesis-related genes, including BmSxl, implying that BmSxl acts downstream of BmPrmt5 and BmVasa to regulate apyrene sperm development. These findings indicate that BmPrmt5 and BmVasa constitute an integral regulatory module essential for spermatogenesis in B. mori. Author summaryIn lepidopteran insects, males produce both nucleated and anucleated sperm. Both sperm morphs are essential for fertilization: The eupyrene sperm fertilizes the egg, and the apyrene sperm assist in transport of the eupyrene sperm to the female sperm-storage organs. In this study, we defined the function of the arginine methyltransferase Prmt5 and its substrate Vasa in the lepidopteran insect Bombyx mori. Our genetic analyses revealed that both BmPrmt5 and BmVasa are essential for spermatogenesis. Combined with RNA-seq, genetic, immunofluorescence staining, and mass spectrometry analyses suggest that the BmPrmt5-BmVasa module regulates spermatogenesis in B. mori. This study expands our knowledge of reproduction biology in insects and identifies potential gene targets for pest control.

Automated summary

In lepidopteran insects, both nucleated and anucleated sperm are essential for fertilization. Prmt5 and Vasa genes play critical roles in regulating spermatogenesis in these insects.