Extraction, Purification, Characterization and Application in Livestock Wastewater of S Sulfur Convertase

Sulfide is a toxic pollutant in the farming environment. Microbial removal of sulfide always faces various biochemical challenges, and the application of enzymes for agricultural environmental remediation has promising prospects. In this study, a strain of Cellulosimicrobium sp. was isolated: numbered strain L1. Strain L1 can transform S2-, extracellular enzymes play a major role in this process. Next, the extracellular enzyme was purified, and the molecular weight of the purified sulfur convertase was about 70 kDa. The sulfur convertase is an oxidase with thermal and storage stability, and the inhibitor and organic solvent have little effect on its activity. In livestock wastewater, the sulfur convertase can completely remove S2-. In summary, this study developed a sulfur convertase and provides a basis for the application in environmental remediation.

Automated summary

This study isolated a Cellulosimicrobium sp. strain, named L1, which can transform sulfide. The extracellular enzyme plays a major role in this process. The purified sulfur convertase has thermal and storage stability, and is not significantly affected by inhibitors and organic solvents. The sulfur convertase can completely remove sulfide in livestock wastewater.