Journal
FRONTIERS IN AGING NEUROSCIENCE
Volume 14, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fnagi.2022.1056067
Keywords
drug discovery; microglia; astrocytes; neuroinflammation; hyperexcitability; triculture; multi-electrode array
Categories
Funding
- Alzheimer's Research UK
- [ARUK-2018DDI-UCL]
- [ARUK-2021DDI-UCL]
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Neuroinflammation and hyperexcitability play important roles in the pathogenesis of neurodegenerative diseases. To study the cellular interactions involved in these processes, a new triculture system containing neurons, astrocytes and microglia was developed. The presence of the intended cell types was confirmed and it was found that microglia suppressed neuronal activity in a dose-dependent manner in the triculture model. This functional triculture model provides a more relevant and complete platform for studying glia-neuron interactions and drug discovery.
Neuroinflammation and hyperexcitability have been implicated in the pathogenesis of neurodegenerative disease, and new models are required to investigate the cellular crosstalk involved in these processes. We developed an approach to generate a quantitative and reproducible triculture system that is suitable for pharmacological studies. While primary rat cells were previously grown in a coculture medium formulated to support only neurons and astrocytes, we now optimised a protocol to generate tricultures containing neurons, astrocytes and microglia by culturing in a medium designed to support all three cell types and adding exogenous microglia to cocultures. Immunocytochemistry was used to confirm the intended cell types were present. The percentage of ramified microglia in the tricultures decreases as the number of microglia present increases. Multi-electrode array recordings indicate that microglia in the triculture model suppress neuronal activity in a dose-dependent manner. Neurons in both cocultures and tricultures are responsive to the potassium channel blocker 4-aminopyridine, suggesting that neurons remained viable and functional in the triculture model. Furthermore, suppressed neuronal activity in tricultures correlates with decreased densities of dendritic spines and of the postsynaptic protein Homer1 along dendrites, indicative of a direct or indirect effect of microglia on synapse function. We thus present a functional triculture model, which, due to its more complete cellular composition, is a more relevant model than standard cocultures. The model can be used to probe glia-neuron interactions and subsequently aid the development of assays for drug discovery, using neuronal excitability as a functional endpoint.
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