Journal
FORESTS
Volume 14, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/f14020215
Keywords
pine wilt disease; dsRNA; TDO gene; white gene; eye pigmentation
Categories
Ask authors/readers for more resources
In this study, a tryptophan 2,3-dioxygenase (TDO) gene related to ommochrome synthesis in insects was identified. Using RNAi technology, the TDO gene of Monochamus alternatus was silenced, resulting in white-eye phenotypes in the pupal and adult stages. The expression of the TDO gene was significantly decreased 48 hours after injection. This discovery is important for future genetic engineering tools to control M. alternatus populations.
Monochamus alternatus Hope (Coleoptera: Cerambycidae), an invasive beetle that has caused billions of dollars of economic losses, is a serious pest of Pinus massoniana in many Asian countries. An efficient RNAi system is helpful for functional genomics research on M. alternatus. In this study, a tryptophan 2,3-dioxygenase (TDO) related to the ommochrome synthesis in insects was identified. Using RNAi technology, the M. alternatus TDO gene was silenced by injecting dsRNA into pupae, and individuals were analyzed by phenotype and expression of the TDO gene by RT-qPCR. The results show that TDO is expressed in different developmental stages of M. alternatus, having its peak expression during the prepupal stage. White-eye phenotypes were observed in the pupal and adult stages after dsRNA injection, and a significant 81% decrease in TDO mRNA levels 48 h after injection was determined by RT-qPCR. This gene can be used as a genetic marker and is an important discovery for future genetic engineering tools to control M. alternatus populations.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available