Journal
CHEMPLUSCHEM
Volume 87, Issue 11, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cplu.202200325
Keywords
aptamer; biosensor; electrochemical; multi-valent; thrombin
Categories
Funding
- National Natural Science Foundation of China
- National Key Research and Development Program of China
- joint NSFC-ISF Research Grant Program
- Natural Science Foundation of Hubei Province
- Zhejiang Provincial Natural Science Foundation of China
- [22090050]
- [21874121]
- [22122410]
- [21804121]
- [22274144]
- [2021YFA1200403]
- [2018YFE0206900]
- [22161142020]
- [2020CFA037]
- [LD21B050001]
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This study reports the design of an E-AB biosensor with dual recognition sites for rapid detection of thrombin. The biosensor showed faster response time and good performance in terms of dynamic range and specificity.
The electrochemical aptamer-based (E-AB) biosensor usually has a long reaction time when detecting thrombin. This work reports the design of an E-AB biosensor with dual recognition sites to quickly detect thrombin. Specifically, two specific recognition sites of thrombin were used to design three aptamer sequences (TBA-15, TBA-29 and TBA-U), followed by fabrication of corresponding sensors. First, we tested these three types of biosensors in tris buffer solution, and found that the response time of the TBA-U sensor to the same concentration of thrombin was about 2 hours, which is shorter than TBA-15 and TBA-29 sensors. Then, we also did the same test in 50 % diluted serum with 500 nM thrombin. The response time of the TBA-U sensor was about 2 hours, which is still faster than the 3 hours of TBA-15 sensor and the 5.5 hours for TBA-29 sensor. In addition, in terms of dynamic range and specificity, TBA-U has good performance.
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