Journal
JOURNAL OF PLANT BIOLOGY
Volume 59, Issue 2, Pages 172-181Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s12374-016-0535-0
Keywords
Glycine max; Gene expression; Low phosphorus; N-2-fixing; Nodule metabolism; Transgene
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Funding
- National Transgene Science and Technology Major Program [2011ZX08004-005, 2013ZX08004-005, 2014ZX08004-005]
- Open Research Fund of the National Key Laboratory of Crop Genetics & Germplasm Enhancement [ZW2013006]
- Priority Academic Program Development of Jiangsu Higher Education Institutions of China [2014PAPD]
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Phosphorus (P) deficiency is one of the major factors that limit legume nodulation and nitrogen (N) fixation, and thus legume productivity. In our previous study, we showed that three T-2 transgenic soybean lines overexpressing rice phosphate transporter gene OsPT2 showed enhanced tolerance to low P stress. This study aimed to determine whether OsPT2 overexpression would increase N-2 fixation and ammonium assimilation in three T-3 homozygous transgenic lines (HTLs) under P deficiency in pot culture. Under low inorganic phosphate (Pi) conditions, the P accumulation, total N and total ureide concentrations were significantly higher in the T-3 HTLs than in the wild type (WT) plants. Further, the T-3 HTLs showed significantly better plant growth performance and nodule development than the WT plants under low-Pi conditions. Quantitative real-time PCR (qRT-PCR) analysis showed that the expression levels of GmENOD40-1, GmENOD40-2 (two early nodulin genes), and GmLba (one leghemoglobin gene) were significantly increased in T-3 HTLs under P deficiency at 24 and 32 d after inoculation (DAI). The increased transcript levels of GmGS1 beta 1 and GmGS1 beta 2 (two cytosolic glutamine synthetase genes) in the T-3 HTLs were consistent with the increase in glutamine synthetase (GS, EC 6.3.1.2) activity at 32 DAI. Our results indicated that the overexpression of OsPT2 in T-3 HTLs enhances N-2 fixation and ammonium assimilation activity under low P stress.
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