Microenvironment-Adaptive Nanozyme for Accelerating Drug-Resistant Bacteria-Infected Wound Healing

Reactive oxygen species (ROS) are favorable for antibacterial infection but their overproduction results in serious inflammatory response and aggravates the hypoxic state of the wound tissue, which is detrimental to healing stages of proliferation and remodeling. Here, an atomic-dispersion Fe-doped oxygen-deficient molybdenum oxide MoO3-X (ADFM) bifunctional nanozyme, featuring implanted peroxidase-like and enhanced catalase-like activity, is developed for decomposing H2O2 into strongly oxidizing hydroxyl radicals (center dot OH) for prevention of bacterial infection and into plentiful O-2 for healing stages. Therein, the introduction of Fe into MoO3-X primarily produces an asymmetric electron density difference by elongating the bond length between metal atoms, synchronously stabilizing adsorption of center dot OH and weakening the adsorption of O-2. ADFM also shows unimaginably high aqueous dispersity and pH-adaptive ROS regulation in the wound microenvironment, both of which are favorable for ADFM to fully exert enzyme-like activity for timely antibacterial and efficient wound-healing action. ADFM thus achieves efficient healing of drug-resistant bacteria-infected wounds in vivo, at an ultralow dosage of 30 mu g mL(-1) against 10(6) CFU mL(-1) extended spectrum beta-lactamases-producing Escherichia coli, exhibiting a wound-healing efficiency of approximate to 10 mm(2) per day, which sets a benchmark among these noble-metal-free nanozyme-based wound-healing agents.

Automated summary

An atomic-dispersion Fe-doped oxygen-deficient molybdenum oxide nanozyme (ADFM) with peroxidase-like and enhanced catalase-like activity is developed for prevention of bacterial infection and promotion of wound healing. ADFM exhibits high aqueous dispersity and pH-adaptive ROS regulation, allowing it to fully exert enzyme-like activity in the wound microenvironment. In vivo experiments show that ADFM achieves efficient healing of drug-resistant bacteria-infected wounds with a wound-healing efficiency of approximately 10 mm(2) per day.