Optimizing in vitro nutrient and ex vitro soil mediums-driven responses for multiplication, rooting, and acclimatization of pineapple

A comprehensive study was carried out on in vitro multiplication and rooting using the medium enriched with different plant growth regulators and acclimatization of pineapple cv. 'Smooth Cayenne' using different soil growing substrates. The significantly highest shoot buds (Avg. 16.7) were obtained on the medium comprising 2.0 mg L-1 BA (6-Benzylaminopurine). Results showed that 1.0 mg L-1 IBA (Indole-3-butyric acid) increased the thickness and length of white adventitious roots and resulted in a significantly highest number of roots (Avg. 8) and root length (6.15 cm). Plantlets with healthy, multiple roots were transplanted in several soil combinations of river silt, bolhari (yellow sand), and peat moss. However, the significantly highest survival (100%) of plantlets in the greenhouse was obtained on the soil medium containing only peat moss. Furthermore, soil mixtures of bolhari and peat moss (1:1) and river silt alone exhibited 98.9% and 95.1% survivability of plantlets, which was also considered equally significant (at 5% probability level). The in vitro and ex vitro protocols optimized in the current study can be applied commercially for pineapple crop worldwide.

Automated summary

A comprehensive study was conducted on in vitro multiplication and rooting of pineapple cv. 'Smooth Cayenne' by using different plant growth regulators. The highest shoot buds were obtained on the medium with 2.0 mg L-1 BA, while 1.0 mg L-1 IBA significantly increased the thickness, length, and number of white adventitious roots. When transplanted, plantlets showed highest survival rates in the medium containing only peat moss, followed by soil mixtures of bolhari and peat moss (1:1) and river silt alone. The optimized protocols in this study can be commercially applied for pineapple cultivation worldwide.