Hydrolyzed Collagen Induces an Anti-Inflammatory Response That Induces Proliferation of Skin Fibroblast and Keratinocytes

Collagen-based products are found in different pharmaceuticals, medicine, food, and cosmetics products for a wide variety of applications. However, its use to prevent or improve the health of skin is growing dizzyingly. Therefore, this study investigated whether collagen peptides could induce fibroblast and keratinocyte proliferation and activation beyond reducing an inflammatory response induced by lipopolysaccharide (LPS). Human skin fibroblasts (CCD-1072Sk) and human keratinocytes (hKT-nh-skp-KT0026) were seeded at a concentration of 5 x 10(4) cells/mL. LPS (10 ng/mL) and three doses of collagen peptides (2.5 mg/mL, 5 mg/mL, 10 mg/mL) were used. The readout parameters were cell proliferation; expression of inducible nitric oxide synthase (iNOS); expression of pro-collagen-1 alpha by fibroblasts; and secretion of interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor alpha (TNF-alpha), transforming growth factor beta (TGF-beta), and vascular endothelial growth factor (VEGF) by both cell types. The results demonstrated that all doses of collagen supplementation induced increased proliferation of both human fibroblasts (p < 0.01) and human keratinocytes (p < 0.001), while only the dose of 10 mg/mL induced an increased expression of pro-collagen-1 alpha by fibroblasts. Similarly, only the dose of 10 mg/mL reduced LPS-induced iNOS expression in fibroblasts (p < 0.05) and keratinocytes (p < 0.01). In addition, collagen supplementation reduced the LPS-induced IL-1 beta (p < 0.05), IL-6 (p < 0.001), IL-8 (p < 0.01), and TNF-alpha (p < 0.05), and increased the TGF-beta and VEGF expression in fibroblasts. Furthermore, collagen supplementation reduced the LPS-induced IL-1 beta (p < 0.01), IL-6 (p < 0.01), IL-8 (p < 0.01), and TNF-alpha (p < 0.001), and increased the TGF-beta (p < 0.05) and VEGF (p < 0.05) expression in keratinocytes. In conclusion, collagen peptides were found to induce fibroblast and keratinocyte proliferation and pro-collagen-1 alpha expression, involving increased expression of TGF-beta and VEGF, as well as the suppression of an inflammatory response induced by LPS.

Automated summary

This study investigated the effects of collagen peptides on the proliferation and activation of fibroblasts and keratinocytes. The results showed that collagen peptides can promote cell proliferation and reduce inflammatory responses caused by lipopolysaccharide.