Journal
NUTRIENTS
Volume 14, Issue 21, Pages -Publisher
MDPI
DOI: 10.3390/nu14214670
Keywords
calorie restriction; food restriction; anxiety-like behaviour; ageing; gene expression; transcriptomics; hypothalamus; amygdala; pituitary; adrenal glands
Categories
Funding
- Epigenes Australia through a La Trobe University Collaborative Research Agreement (LTU project) [20115]
- Oracle for Research Grant [CPQ-2618253]
- National Collaborative Research Infrastructure Strategy (NCRIS)
- Pawsey Supercomputing Centre
- Australian Government
- Government of Western Australia
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The study found that long-term calorie restriction can reduce anxiety-like behavior at different stages and identified a transcriptome signature of long-term calorie restriction.
Further examination of the molecular regulators of long-term calorie restriction (CR), reported to have an anxiolytic effect, may highlight novel therapeutic targets for anxiety disorders. Here, adult male Hooded Wistar rats were exposed to a 25% CR whilst anxiety-like behaviour was assessed at 6-, 12-, and 18-months of age via the elevated plus maze, open field, and acoustic startle tests. Next-generation sequencing was then used to measure transcriptome-wide gene expression in the hypothalamus, amygdala, pituitary, and adrenal glands. Results showed an anxiolytic behavioural profile across early, middle, and late adulthood by CR, with the strongest effects noted at 6-months. Transcriptomic analysis by seven attribute weighting algorithms, including Info Gain Ratio, Rule, Chi Squared, Gini Index, Uncertainty, Relief, and Info Gain, led to the development of a signature of long-term CR, independent of region. Complement C1q A chain (C1qa), an extracellular protein, expression was significantly decreased by CR in most regions examined. Furthermore, text mining highlighted the positive involvement of C1qa in anxiety, depression, neurodegeneration, stress, and ageing, collectively identifying a suitable biomarker candidate for CR. Overall, the current study identified anxiety-related phenotypic changes and a novel transcriptome signature of long-term CR, indicating potential therapeutic targets for anxiety, depression, and neurodegeneration.
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