Cytotoxic CD8+ T cells target citrullinated antigens in rheumatoid arthritis

The immune mechanisms that mediate synovitis and joint destruction in rheumatoid arthritis (RA) remain poorly defined. Although increased levels of CD8(+) T cells have been described in RA, their function in pathogenesis remains unclear. Here we perform single cell transcriptome and T cell receptor (TCR) sequencing of CD8(+) T cells derived from anti-citrullinated protein antibodies (ACPA)+ RA blood. We identify GZMB(+)CD8(+) subpopulations containing large clonal lineage expansions that express cytotoxic and tissue homing transcriptional programs, while a GZMK(+)CD8(+) memory subpopulation comprises smaller clonal expansions that express effector T cell transcriptional programs. We demonstrate RA citrullinated autoantigens presented by MHC class I activate RA blood-derived GZMB(+)CD8(+) T cells to expand, express cytotoxic mediators, and mediate killing of target cells. We also demonstrate that these clonally expanded GZMB(+)CD8(+) cells are present in RA synovium. These findings suggest that cytotoxic CD8(+) T cells targeting citrullinated antigens contribute to synovitis and joint tissue destruction in ACPA+ RA. The immune mechanisms underlying synovitis and joint tissue destruction in rheumatoid arthritis (RA) remain incompletely defined. Here, the authors demonstrate that ACPA+ RA patients have activated clonally expanded cytotoxic GZMB+ CD8+ T cells in blood and synovium that target and are activated by citrullinated antigens to mediate cell killing.

Automated summary

This study reveals that cytotoxic CD8(+) T cells targeting ACPA are involved in synovitis and joint tissue destruction in rheumatoid arthritis (RA), contributing to the understanding of the immune mechanisms underlying RA pathogenesis.