ARID1A loss induces polymorphonuclear myeloid-derived suppressor cell chemotaxis and promotes prostate cancer progression

The accumulation of myeloid derived suppressor cells (MDSC) has been associated with prostate cancer progression and castration resistance. Here the authors show that loss of ARID1A, a subunit of the SWI/SNF chromatin remodeling complex, results in polymorphonuclear-MDSC infiltration and cooperates with Pten loss to accelerate prostate tumorigenesis. Chronic inflammation and an immunosuppressive microenvironment promote prostate cancer (PCa) progression and diminish the response to immune checkpoint blockade (ICB) therapies. However, it remains unclear how and to what extent these two events are coordinated. Here, we show that ARID1A, a subunit of the SWI/SNF chromatin remodeling complex, functions downstream of inflammation-induced IKK beta activation to shape the immunosuppressive tumor microenvironment (TME). Prostate-specific deletion of Arid1a cooperates with Pten loss to accelerate prostate tumorigenesis. We identify polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) as the major infiltrating immune cell type that causes immune evasion and reveal that neutralization of PMN-MDSCs restricts the progression of Arid1a-deficient tumors. Mechanistically, inflammatory cues activate IKK beta to phosphorylate ARID1A, leading to its degradation via beta-TRCP. ARID1A downregulation in turn silences the enhancer of A20 deubiquitinase, a critical negative regulator of NF-kappa B signaling, and thereby unleashes CXCR2 ligand-mediated MDSC chemotaxis. Importantly, our results support the therapeutic strategy of anti-NF-kappa B antibody or targeting CXCR2 combined with ICB for advanced PCa. Together, our findings highlight that the IKK beta/ARID1A/NF-kappa B feedback axis integrates inflammation and immunosuppression to promote PCa progression.

Automated summary

Loss of ARID1A promotes prostate cancer progression and immunosuppression by promoting the infiltration of polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs). Inflammatory cues activate IKK beta to phosphorylate ARID1A and degrade it, leading to CXCR2 ligand-mediated PMN-MDSC chemotaxis. Neutralization of PMN-MDSCs restricts the progression of ARID1A-deficient tumors.