Journal
NATURE COMMUNICATIONS
Volume 13, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41467-022-34860-y
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Funding
- Discovery Projects funding scheme [DP200100624]
- NHMRC [APP2010154]
- Jack Brockhoff foundation (JBF) [4659-2019]
- St Vincent's Hospital (Melbourne) Research Endowment Fund
- Stafford Fox Medical Research Foundation
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This study presents a method using a soft resistive force-sensing diaphragm to monitor the contraction forces and beating patterns of cardiac organoids in real-time. The method overcomes the mismatch between the topological complexity and soft tissue properties of organoids and rigid force sensors, providing a new approach for studying the mechanical properties of cardiac tissues.
Time-lapse mechanical properties of stem cell derived cardiac organoids are important biological cues for understanding contraction dynamics of human heart tissues, cardiovascular functions and diseases. However, it remains difficult to directly, instantaneously and accurately characterize such mechanical properties in real-time and in situ because cardiac organoids are topologically complex, three-dimensional soft tissues suspended in biological media, which creates a mismatch in mechanics and topology with state-of-the-art force sensors that are typically rigid, planar and bulky. Here, we present a soft resistive force-sensing diaphragm based on ultrasensitive resistive nanocracked platinum film, which can be integrated into an all-soft culture well via an oxygen plasma-enabled bonding process. We show that a reliable organoid-diaphragm contact can be established by an 'Atomic Force Microscope-like' engaging process. This allows for instantaneous detection of the organoids' minute contractile forces and beating patterns during electrical stimulation, resuscitation, drug dosing, tissue culture, and disease modelling.
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