4.6 Article

Fission yeast cells mix parental mitochondria in a progressive manner during meiosis

Journal

JOURNAL OF MOLECULAR CELL BIOLOGY
Volume 14, Issue 11, Pages -

Publisher

OXFORD UNIV PRESS
DOI: 10.1093/jmcb/mjac070

Keywords

mitochondria; mitochondrial inheritance; mitochondrial fission; fission yeast

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This study used live-cell microscopy and an algorithm to quantitatively assess the mixing of parental mitochondria in fission yeast. The results showed that parental mitochondria mixed progressively during meiosis. The study also identified mitochondrial fission and the size of the conjugation neck as limiting factors in restricting mitochondrial mixing.
Mitochondria in many fungi are inherited uniparentally during meiosis. It has remained unclear whether parental mitochondria in the fission yeast Schizosaccharomyces pombe are inherited uniparentally or biparentally. Here, we assessed the mixing of parental mitochondria carefully by live-cell microscopy and developed an algorithm to determine the degree of mitochondrial mixing in a quantitative manner. We found that parental mitochondria in fission yeast cells were mixed progressively as meiosis progressed. Moreover, we established that mitochondrial fission and the size of the conjugation neck are the limiting factors in restricting the mixing of parental mitochondria. We further employed a combination of quantitative polymerase chain reaction, fluorescent live-cell microscopy, and transmission electron microscopy approaches to examine the mitochondrial inheritance of progeny cells derived from a cross between wild-type and Rho0 (mitochondrial DNA absent) cells. The results show that all progeny cells of the cross carry mitochondrial DNA. Hence, our data support the model in which parental mitochondria in the fission yeast S. pombe are inherited biparentally during meiosis.

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