Journal
VIRUSES-BASEL
Volume 14, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/v14122678
Keywords
Cryphonectria hypovirus 1; England; transmissions; preservations; seedlings; branches; concentration; compatibility; real-time PCR
Categories
Funding
- Department for Environment, Food Rural Affairs
- [TH0157]
- [TH3_1]
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Biological control of chestnut blight using virus infection (hypovirulence) has been effective in Europe and parts of North America. In this study, two strains of CHV-1 virus showed great biocontrol potential and could convert virulent cankers to hypovirulent ones. Additionally, a newly developed real-time PCR method was introduced to detect and quantify CHV-1 virus.
Biological control of Cryphonectria parasitica fungus, causal agent of chestnut blight, by virus infection (hypovirulence) has been shown to be an effective control strategy against chestnut blight in Europe and some parts of North America. The most studied mycovirus is the Cryphonectria hypovirus 1 (CHV-1) type species of the Hypoviridae family. To efficiently provide biocontrol, the virus must be able to induce hypovirulence in its fungal host in chestnut trees. Here, two different CHV-1 subtype I virus strains (E-5 and L-18), gained by transmissions, were tested for their hypovirulence induction, biocontrol potential, and transmission between vegetatively compatible (VCG) and incompatible fungal isolate groups in sweet chestnut seedlings and branches. Both strains of CHV-1 showed great biocontrol potential and could protect trees by efficiently transmitting CHV-1 by hyphal anastomosis between fungal isolates of the same VCG and converting virulent to hypovirulent cankers. The hypovirulent effect was positively correlated with the virus concentration, tested by four different reverse-transcription PCRs, two end-point and two real-time methods, one of which represents a newly developed real-time PCR for the detection and quantification of CHV-1.
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