Journal
VIRUSES-BASEL
Volume 14, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/v14112500
Keywords
Riptortus pedestris; insect-specific virus; metatranscriptomic sequencing; small interference RNA
Categories
Funding
- Ningbo Public Welfare Technology Plan Project [2021S012]
- Science Foundation of Zhejiang Province [LQ21C140004]
- China National Novel Transgenic Organisms Breeding Project [2019ZX08004-004]
- Ningbo Science and Technology Innovation 2025 Major Project [2019B10004]
- K. C. Wong Magna Fund in Ningbo University
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This study collected Riptortus pedestris from six soybean-producing regions in China and used metatranscriptomic approach to analyze their virome. Seven new insect RNA viruses were identified, three of which had complete genome sequences. These newly identified viruses belonged to the family Rhabdoviridae and provided viral information for the development of potential soybean insect-specific biocontrol agents.
Insect-specific virus (ISV) is one of the most promising agents for the biological control of insects, which is abundantly distributed in hematophagous insects. However, few ISVs have been reported in Riptortus pedestris (Fabricius), one of the major pests threatening soybeans and causing great losses in yield and quality. In this work, field Riptortus pedestris was collected from six soybean-producing regions in China, and their virome was analyzed with the metatranscriptomic approach. Altogether, seven new insect RNA viruses were identified, three of which had complete RNA-dependent RNA polymerase (RdRp) and nearly full-length genome sequences, which were named Riptortus pedestris alphadrosrha-like virus 1 (RpALv1), Riptortus pedestris alphadrosrha-like virus 2 (RpALv2) and Riptortus pedestris almendra-like virus (RiALv). The three identified novel ISVs belonged to the family Rhabdoviridae, and phylogenetic tree analysis indicated that they were clustered into new distinct clades. Interestingly, the analysis of virus-derived small-interfering RNAs (vsiRNAs) indicated that only RiALv-derived siRNAs exhibited 22 nt length preference, whereas no clear 21 or 22 nt peaks were observed for RpALv1 and RpALv2, suggesting the complexity of siRNA-based antiviral immunity in R. pedestris. In conclusion, this study contributes to a better understanding of the microenvironment in R. pedestris and provides viral information for the development of potential soybean insect-specific biocontrol agents.
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