Effects of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Infection on the Surface Glycoprofiling of Porcine Pulmonary Microvascular Endothelial Cells

Article Biochemical Research Methods

Isolation and culture of rat intestinal mucosal microvascular endothelial cells using immunomagnetic beads

Yanmei Wu et al.

Summary: In this study, a method using magnetic beads was established to isolate highly pure MVECs from rat intestinal mucosae. Magnetic separation may influence the expression of FVIII.

JOURNAL OF IMMUNOLOGICAL METHODS (2022)

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Review Cell Biology

Vascular Endothelial Glycocalyx Damage and Potential Targeted Therapy in COVID-19

Duoduo Zha et al.

Summary: COVID-19 is a highly infectious respiratory disease caused by SARS-CoV-2. Endothelial cells and the glycocalyx layer play a central role in the pathogenesis of COVID-19. Inflammatory mediators, reactive oxygen species, matrix metalloproteases, glycocalyx fragments, and viral proteins may contribute to endothelial glycocalyx damage in COVID-19. Potential therapeutic strategies targeting the glycocalyx layer are discussed.

CELLS (2022)

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Article Virology

IFIT3 and IFIT5 Play Potential Roles in Innate Immune Response of Porcine Pulmonary Microvascular Endothelial Cells to Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus

Yanmei Wu et al.

Summary: The roles of IFIT3 and IFIT5 in inhibiting HP-PRRSV replication in porcine pulmonary MVECs were investigated, and the effects of astragalus polysaccharides (APS) on them were studied. It was found that IFIT3 and IFIT5 play an important role in inhibiting HP-PRRSV replication, and APS suppresses the multiplication of the virus by upregulating their expression.

VIRUSES-BASEL (2022)

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Article Peripheral Vascular Disease

Microvascular dysfunction in COVID-19: the MYSTIC study

Alexandros Rovas et al.

Summary: Our study found that COVID-19 patients have reduced microvascular density, slower red blood cell velocity, and glycocalyx damage. Elevated markers of endothelial dysfunction are associated with disease severity and can predict 60-day in-hospital mortality.

ANGIOGENESIS (2021)

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Article Biochemical Research Methods

Quantitative Proteomic Analysis of Global Protein Acetylation in PRRSV-Infected Pulmonary Alveolar Macrophages

Jianyu Fang et al.

Summary: This study identified 3731 lysine acetylation sites on 1421 cellular proteins in PRRSV-infected PAMs, indicating the involvement of these acetylated proteins in host immune responses and energy metabolism. These findings will help in understanding PRRSV pathogenesis and identifying new cellular targets for anti-PRRSV therapeutics.

PROTEOMICS (2021)

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Article Hematology

Vascular Endothelial Damage in the Pathogenesis of Organ Injury in Severe COVID-19

Annabelle Dupont et al.

Summary: The study investigated the association between endothelial damage and immune dysregulation with organ failure, thrombus formation, and death in 82 patients with COVID-19, finding a direct role of endotheliopathy in organ failure and an association between dysregulated immune response and respiratory failure, liver injury, and death. Thrombi from COVID-19 patients on extracorporeal membrane oxygenation showed increased neutrophil accumulation and neutrophil extracellular traps compared to non-COVID-19 thrombi.

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY (2021)

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Review Critical Care Medicine