Journal
VIROLOGY
Volume 578, Issue -, Pages 163-170Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2022.12.007
Keywords
Simian immunodeficiency virus; Gag polyprotein; Nucleocapsid protein; Virion assembly; Viral RNA packaging
Categories
Ask authors/readers for more resources
Mutations C33S and R7A/K8A impair virion assembly and viral RNA binding, while mutations C12S and R29A/R30A allow substantial particle production and genomic RNA encapsidation. These findings help define the relative contribution of the SIV NC zinc finger motifs and basic regions to the NC biological properties.
The nucleocapsid (NC) domain of the retroviral Gag polyproteins mediates the incorporation of the viral genomic RNA into virions. Although SIV is widely used as a model for human immunodeficiency virus type 1 (HIV-1) infections, the SIV NC has been the subject of few studies which have provided discrepant data on the relative contribution of the two NC zinc finger motifs to genomic RNA encapsidation. Here, we demonstrate that mu-tations affecting the first cysteine in the distal zinc finger motif (C33S) or the N-terminal NC basic domain (R7A/ K8A) drastically impair virion assembly and viral RNA binding. By contrast, amino acid substitutions targeting the first cysteine of the proximal zinc finger (C12S) or the basic region connecting both zinc fingers (R29A/R30A) allow substantial particle production and genomic RNA encapsidation. Our results help define the relative contribution of the SIV NC zinc finger motifs and basic regions to the NC biological properties.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available