Electric yo-yo centrifugation combining with paper-based microfluidic immunoassay chip for inflammatory biomarkers detection in whole blood

At present, most countries or regions use commercial centrifuges for centrifugation, but this is out of reaching for limited-resource areas. To overcome this problem, a portable electric yo-yo as centrifuge was firstly proposed to obtain serum, and this device can be combined with paper-based analytical devices for enzyme-linked immu-nosorbent assay (ELISA) analysis from human whole blood. In this study, inflammatory biomarkers C-reactive protein (CRP) and serum amyloid A (SAA) were used as target biomarker to verify the performance of the proposed method. The results shows good performance and their detection limits were determined to be 580 pg/ mL for CRP and 800 pg/mL for SAA, respectively. We believe this method provides a new platform of low cost and fast detection for inflammatory biomarkers in the limited-resource settings.

Automated summary

In this study, a portable electric yo-yo was proposed as a centrifuge for obtaining serum from human whole blood. It can be combined with paper-based analytical devices for the detection of inflammatory biomarkers. The method shows good performance and provides a low-cost and fast detection platform in limited-resource settings.