Fabrication of novel electroactive nickel sulfide@graphene oxide nanocomposite integrated transduction platform for non-enzymatic electrochemical sensing of Hydrogen Peroxide in environmental and biological samples

Hydrogen Peroxide (H2O2) is an imperative molecule in biochemical reactions yet at the same time fairly injurious in surplus. Developing an affordable, stable and sensitive analytical method capable of sensing H2O2 is still challenging. Herein, a novel sensing platform was fabricated based on highly electroactive, non-enzymatic and stable nanocomposite NiS@GO for detection of H2O2. The physico-chemical properties of the nanocomposite were analyzed by UV-Vis spectroscopy, SEM, FESEM, FT-IR, Raman spectroscopy and XRD. The synergetic effect between NiS and GO enhanced the charge transportation ability of nanocomposite NiS@GO allowed a liable electrochemical response (generally assured at 0.48 V vs Ag/AgCl2) that lessen in the presence of H2O2. The reduction in current was used for the quantitative measurement of H2O2. The influence of various factors on the response behavior of the electrodes was probed and optimized. After comprehensive optimization, the LOD was calculated at a linear range of 0.1 mu M to 1 mM (R-2 = 0.988) and found to be as low as 0.059 mu M. Moreover, the proposed sensor was practiced with real samples i.e. tap water and milk, and the acquired outcome (recoveries ranging, 91-99 %) confirmed the exceptional catalytic exposition with good sensitivity and selectivity for quantification and qualitative of H2O2. Hence, this type of non-enzymatic sensor can be devised into portable one for onsite monitoring of environmental, human health, and other arenas.

Automated summary

Researchers have developed a novel sensing platform based on a nanocomposite NiS@GO for the detection of hydrogen peroxide (H2O2). The sensor exhibits high electroactivity, non-enzymatic stimulation, and stability, allowing for sensitive detection of H2O2. After optimization, the sensor has a limit of detection (LOD) of 0.059 μM in a linear range of 0.1 μM to 1 mM (R-2 = 0.988), demonstrating good sensitivity and selectivity.