Journal
CELL BIOCHEMISTRY AND BIOPHYSICS
Volume 72, Issue 2, Pages 605-610Publisher
HUMANA PRESS INC
DOI: 10.1007/s12013-014-0508-1
Keywords
Microfluidic chip; Surface modification; NOA 61
Funding
- National Natural Science Foundation of China [51375024]
- Scientific-technical project of Beijing Education Committee [KZ201210005009]
- opening foundation of the State Key Laboratory of Space Medicine Fundamentals and Application, Chinese Astronaut Research and Training Center [SMFA13K06]
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In this paper, we report the improvement of PCR microfluidic chip efficiency achieved by coating the inner surface of steel capillary microchannel with a 22-A mu m film of the ultraviolet-solidified NOA61 using a device invented by us. Our results indicate that with this treatment, the roughness of the inside wall of steel capillary was improved from Ra = 0.921 to Ra = 0.254. The contact angle was decreased from about 95A degrees to 56A degrees, and the surface hydrophobicity was also increased. The flow pressure for performing the real-time PCR in the microfluidic chip with modified surface was reduced by twofold (2.11/1) and that resulted in a substantially increased efficiency of PCR. A modification of the microchannel interior surface improved the quality of the on-chip integrated PCR procedure.
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