Smart sensory polymer for straightforward Zn(II) detection in pet food samples

We report on an innovative method to measure the Zn(II) concentration in commercial pet food samples, both wet and dry food. It is based on a colorimetric sensory polymer prepared from commercial monomers and 0.5 % of a synthetic monomer having a quinoline sensory core (N-(8-(2-azidoacetamido)quinolin-5-yl)meth-acrylamide). We obtained the sensory polymer as crosslinked films by thermally initiated bulk radical poly-merization of the monomers of 100 mu m thickness, which we punched into o6 mm sensory discs. The immersion of the discs in water solutions containing Zn(II) turned the fluorescence on, allowing for the titration of this cation using the G parameter of a digital picture taken to the discs. The limits of detection and quantification were 29 and 87 mu g/L, respectively. Furthermore, we measured the concentration of Zn(II) even in the presence of other cations, detecting no significant interferences. Thus, in a further step, we obtained the concentration of Zn (II) from 15 commercial pet food samples, ranging from 19 to 198 mg/kg, following a simple extraction pro-cedure and contacting the extractant with our sensory discs. These results were contrasted with that obtained by ICP-MS as a reference method.

Automated summary

We report an innovative method for measuring Zn(II) concentration in commercial pet food samples. This method utilizes a colorimetric sensory polymer and fluorescence titration to accurately measure Zn(II) even in the presence of other cations. The concentration of Zn(II) in 15 samples of commercial pet food was successfully determined using a simple extraction process.