DNA-based assemblies with bischromophoric styryl dye-chromene conjugates and cucurbit[7]uril

Novel conjugates consist of 4-styrylpyridinium dye and 2,2-diphenyl-2H-chromene moiety were obtained, and their affinity to double stranded DNA and cucurbit[7]uril was investigated. With a combination of absorption, fluorescence and circular dichroism spectroscopies as well as MALDI-TOF mass spectrometry, we demonstrate that these compounds can interact with macromolecules to form of the supramolecular assemblies due to two suitable binding sites. The ternary complex is formed as a result of the intercalation of a positively charged styryl part between DNA base pairs, while cucurbit[7]uril is located on the alkyl chain between two moieties of conjugate. All these findings provide valuable information into controlling the interaction between organic molecules, DNA and cucurbit[7]uril.

Automated summary

Novel conjugates consisting of 4-styrylpyridinium dye and 2,2-diphenyl-2H-chromene moiety were synthesized and their interaction with double stranded DNA and cucurbit[7]uril was investigated. The compounds were found to form supramolecular assemblies with the macromolecules through suitable binding sites, which were confirmed by various spectroscopic and mass spectrometric techniques.