Self-Boosting Vaccination Based on Pulsatile Antigen Release from Core-Shell Microparticles

Vaccination is among the most effective ways to prevent infectious diseases. Subunit vaccines are safe but usually require multiple booster shots, which may lead to immunity loss and economic consume. In this study, a self-boosting vaccine is developed based on the pulsatile release of antigen from the core-shell microparticle after single-injection immunization. Self-healing technology applied to form an antigen core can avoid organic solvents from destroying the spatial structure of the antigen. The antigen shell is built-up by self-assemble of the antigen with the opposite charged polypeptide. Primary immunization occurs with the self-assembled film disintegration, and the booster comes with the microparticle degradation. The changing of antigen-specific antibodies after immunization with the core-shell microparticle vaccine is consistent with that caused by the two shots of immunization. The immune effect and safety evaluation results support the translational potential of this self-boosting core-shell microparticle vaccine.

Automated summary

A self-boosting vaccine has been developed in this study, which utilizes pulsatile release of antigen from core-shell microparticles after a single injection. The self-healing technology used for the antigen core formation prevents organic solvents from damaging the spatial structure. The antigen shell is formed through self-assembly of antigen with an oppositely charged polypeptide. The immune effect and safety evaluation results support the translational potential of this self-boosting core-shell microparticle vaccine.