Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 372, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2022.132673
Keywords
Biomembrane; Tenascin-C; Fibronectin; Circulating exosomes; Surface plasmon resonance
Funding
- National Natural Science Foundation of China [61875114]
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In this paper, a biomimetic probe was synthesized by functionalizing cancer cell membrane with gold nanoparticles, achieving highly sensitive detection of circulating exosomes. This strategy offers new possibilities for clinical non-invasive cancer diagnosis.
Circulating exosomes (crEVs) have attracted increasing interest in non-invasive cancer diagnosis and monitoring of treatment response. In this paper, cancer cell membrane functionalized AuNPs (CCM@AuNPs) containing overexpressed Tenascin-C (TNC) were synthesized as a biomimetic probe for crEVs. crEVs containing fibronectin 1 (FN1) could be recognized through the specific interaction between FN1 and TNC. CCM@AuNPs were immobilized on the gold chip surface to decrease nonspecific interactions and enhance the surface plasmon resonance signal to ensure the sensor's accuracy and specificity. The ultra-sensitive crEVs detection was achieved through CCM@AuNPs with a low detection limit of 18.1 particles/mL 1 and the linear range of 3 x 10(4)similar to 3 x 107 particles mL 1. Owing to its simple and direct detection of crEVs, this biomimetic strategy for detecting crEVs offers new possibilities for clinical non-invasive cancer diagnosis.
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