Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 373, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2022.132732
Keywords
ICT; PH probe; Mitochondria-targeted; Cell imaging; Water samples
Funding
- Natural Science Foundation of Shan- dong Province
- National Natural Science Foundation of China
- Scientific Research and Innovation Project for College Students in Shandong Province
- Research Center for Structural Composition and Physical Properties of Shandong University
- [ZR2021MB009]
- [31871407]
- [31870831]
- [202110422059]
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A novel fluorescent probe cys-pH based on intramolecular charge transfer mechanism is developed for pH detection targeting mitochondria, with excellent mitochondrial targeting and sensitive response to fluctuations in mitochondrial pH.
A novel fluorescent probe (cys-pH) based on intramolecular charge transfer (ICT) mechanism for pH detection targeting mitochondria was developed. cys-pH has excellent mitochondrial targeting, a suitable pKa (8.53), a good linear range in the pH range of 6.50-10.00, and a sensitive response to fluctuations in mitochondrial pH. cys-pH has been succeeded in applying to monitor the pH of mitochondria in living cells and the pH detection of water samples in environmental systems. In addition, pH test papers and standard colorimetric cards of cys-pH are prepared. We reasonably explained the double absorption peak of cys-pH UV-vis spectrum in the long wavelength region.
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