The microbial synergy and response mechanisms of hydrolysis-acidification combined microbial electrolysis cell system with stainless-steel cathode for textile-dyeing wastewater treatment

Microbial electrolysis cell (MEC) has been existing problems such as poor applicability to real wastewater and lack of cost-effective electrode materials in the practical application of refractory wastewater. A hydrolysis-acidification com-bined MEC system (HAR-MECs) with four inexpensive stainless-steel and conventional carbon cloth cathodes for the treatment of real textile-dyeing wastewater, which was fully evaluated the technical feasibility in terms of parameter optimization, spectral analysis, succession and cooperative/competition effect of microbial. Results showed that the optimum performance was achieved with a 12 h hydraulic retention time (HRT) and an applied voltage of 0.7 V in the HAR-MEC system with a 100 mu m aperture stainless-steel mesh cathode (SSM-100 mu m), and the associated optimum BOD5/COD improvement efficiency (74.75 +/- 4.32 %) and current density (5.94 +/- 0.03 A center dot m-2) were increased by 30.36 % and 22.36 % compared to a conventional carbon cloth cathode. The optimal system had effective removal of refractory organics and produced small molecules by electrical stimulation. The HAR segment could greatly allevi-ate the imbalance between electron donors and electron acceptors in the real refractory wastewater and reduce the treatment difficulty of the MEC segment, while the MEC system improved wastewater biodegradability, amplified the positive and specific interactions between degraders, fermenters and electroactive bacteria due to the substrate complexity. The SSM-100 mu m-based system constructed by phylogenetic molecular ecological network (pMEN) exhib-ited moderate complexity and significantly strong positive correlation between electroactive bacteria and fermenters. It is highly feasible to use HAR-MEC with inexpensive stainless-steel cathode for textile-dyeing wastewater treatment.

Automated summary

A hydrolysis-acidification combined MEC system with inexpensive stainless-steel and carbon cloth cathodes was used for the treatment of real textile-dyeing wastewater. The system showed promising results in terms of parameter optimization, spectral analysis, microbial succession, and removal of refractory organics.