Remediation of chromium, zinc, arsenic, lead and antimony contaminated acidic mine soil based on Phanerochaete chrysosporium induced phosphate precipitation

Microbial induced phosphate precipitation (MIPP) is an advanced bioremediation technology to reduce the mobility and bioavailability of heavy metals (HMs), but the high level of HMs would inhibit the growth of phosphate solubiliz-ing microbes. This study proposed a new combination system for the remediation of multiple HMs contaminated acidic mine soil, which included hydroxyapatite (HAP) and Phanerochaete chrysosporium (P. chrysosporium, PC) that had high phosphate solubilizing ability and HMs tolerance. Experimental data suggested that in HAP/PC treatment after 35 d of remediation, labile Cr, Zn and As could be transformed into the stable fraction with the maximum immobilization ef-ficiencies increased by 53.01 %, 22.43 %, and 35.65 %, respectively. The secretion of organic acids by P. chrysosporium was proved to promote the dissolution of HAP. Besides, the pH value, available phosphorus (AP) and organic matter (OM) increased in treated soil than in original soil, which also indicated the related dissolution-precipitation mecha-nism of HMs immobilization. Additionally, characterization results revealed that adsorption and ion exchange also played an important role in the remediation process. The overall results suggested that applying P. chrysosporium coupled with HAP could be considered as an efficient strategy for the remediation of multiple HMs contaminated mine soil and laid a foundation for the future exploration of soil microenvironment response during the remediation process.

Automated summary

This study proposed a new combination system for the remediation of multiple heavy metal (HM) contaminated acidic mine soil. Experimental data suggested that the combination of hydroxyapatite (HAP) and Phanerochaete chrysosporium (PC) could effectively transform labile HM into stable fractions with increased immobilization efficiency. The secretion of organic acids by PC promoted the dissolution of HAP, and the pH value, available phosphorus (AP) and organic matter (OM) increased in the treated soil. Adsorption and ion exchange also played important roles in the remediation process.