Journal
CELL AND TISSUE RESEARCH
Volume 360, Issue 1, Pages 129-141Publisher
SPRINGER
DOI: 10.1007/s00441-015-2144-5
Keywords
Light sheet-based fluorescence microscopy (LSFM); Digitally scanned light sheet-based microscopy; Single/selective-plane illumination microscopy; Three-dimensional cell cultures; Cellular spheroids; High-throughput LSFM
Categories
Funding
- Cluster of Excellence for Macromolecular Complexes (CEF-MC) [EXC-115]
- German Ministry for Education and Research (BMBF)
- German Ministry for Education and Research (Forschungsschwerpunkt Biophotonik IV)
- German Ministry for Education and Research (Project ProMEBS)
Ask authors/readers for more resources
In light sheet-based fluorescence microscopy (LSFM), only the focal plane is illuminated by a laser light sheet. Hence, only the fluorophores within a thin volume of the specimen are excited. This reduces photo-bleaching and photo-toxic effects by several orders of magnitude compared with any other form of microscopy. Therefore, LSFM (aka single/selective-plane illumination microscopy [SPIM] or digitally scanned light sheet microscopy [DSLM]) is the technique of choice for the three-dimensional imaging of live or fixed and of small or large three-dimensional specimens. The parallel recording of millions of pixels with modern cameras provides an extremely fast acquisition speed. Recent developments address the penetration depth, the resolution and the recording speed of LSFM. The impact of LSFM on research areas such as three-dimensional cell cultures, neurosciences, plant biology and developmental biology is increasing at a rapid pace. The development of high-throughput LSFM is the next leap forward, allowing the application of LSFM in toxicology and drug discovery screening.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available