Nanobioconjugated System Formed of Folic Acid-Deferrioxamine-Ga(III) on Gold Surface: Preparation, Characterization, and Activities for Capturing of Mouse Breast Cancer Cells 4T1

The folic acid-deferrioxamine B-gallium(III) system was assembled on the goldmercaptopropionic acid surface through an effective method for the first time; then, the assembled nanobioconjugated system, Au-MPA-FOA-DFO-Ga(III), was successfully tested for capturing of the mouse breast cancer cells 4T1. Physicochemical characteristics of the constructed system were studied by attenuated total reflectance Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, cyclic and differential pulse voltammetry, and electrochemical impedance spectroscopy (EIS). To evaluate the capturing ability of the folate receptor (FR) expressed cancer cells by the prepared system, the 4T1 cells were tested as a model of FR-expressed cells, and the human foreskin fibroblast cells were tested as a model of no FR-expressed cells. The presence of cancer cells on the system surface was successfully detected by EIS based on variations of the charge-transfer resistance (R-ct) of the [Fe(CN)(6)](3-/4-) redox probe at the Au-MPA-FOA-DFO-Ga(III) electrode system/solution interface. Large variations observed in the R-ct of the electrode, from 30.26 +/- 0.04 to 227.50 +/- 0.02 k Omega, supported the high affinity of the Au-MPA-FOA-DFO-Ga(III) system for 4T1 cells. Accumulation of the 4T1 cells onto the system surface was found to be time-dependent. The modified electrode exhibited rapid uptake kinetics for 4T1 cells with a t(1/2) of similar to 8.5 min. The experimental results are presented and discussed in this paper.