Journal
CELL AND TISSUE RESEARCH
Volume 364, Issue 1, Pages 95-103Publisher
SPRINGER
DOI: 10.1007/s00441-015-2310-9
Keywords
ACPT; Localization; Odontoblasts; Proliferation; Differentiation
Categories
Funding
- Chonbuk National University
- National Research Foundation of Korea (NRF) - Korean government (MSIP) [2013R1A2A1A01007642, 2014R1A2A1A11049931, 2014R1A1A2056700]
- National Research Foundation of Korea [2013R1A2A1A01007642, 2014R1A2A1A11049931, 2014R1A1A2056700] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Odontoblasts differentiate from dental mesenchyme during dentin formation and mineralization. However, the molecular mechanisms controlling odontoblast differentiation remain poorly understood. Here, we show that expression of testicular acid phosphatase (ACPT) is restricted in the early stage of odontoblast differentiation in proliferating dental mesenchymal cells and secretory odontoblasts. ACPT is expressed earlier than tissue-nonspecific alkaline phosphatase (TNAP) and partly overlaps with TNAP in differentiating odontoblasts. In MDPC-23 odontoblastic cells, expression of ACPT appears simultaneously with a decrease in beta-catenin activity and is abolished with the expression of Phex and Dsp. Knockdown of ACPT in MDPC-23 cells stimulates cell proliferation together with an increase in active beta-catenin and cyclin D1. In contrast, the overexpression of ACPT suppresses cell proliferation with a decrease in active beta-catenin and cyclin D1. Expression of TNAP, Osx, Phex and Dsp is reduced by knockdown of ACPT but is enhanced by ACPT overexpression. When ACPT is blocked with IgG, alkaline phosphatase activity is inhibited but cell proliferation is unchanged regardless of ACPT expression. These findings suggest that ACPT inhibits cell proliferation through beta-catenin-mediated signaling in dental mesenchyme but elicits odontoblast differentiation and mineralization by supplying phosphate during dentin formation. Thus, ACPT might be a novel candidate for inducing odontoblast differentiation and mineralization for dentin regeneration.
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