The relationship of rheological properties and the performance of silk fibroin hydrogels in tissue engineering application

Hydrogel-based systems are widely used for conventional 3D-cell culture, where cells can be seeded on or embedded in 3D-matrix gels for cultivation. Several new approaches have emerged to develop innovative more performing polymeric biomaterials for tissue regeneration. Within this class of biomaterials, land and marine based-polymers (including among other collagens, silk, chitosan/chitin and alginates) have been explored to date. The best-known example of silk to date is the fiber produced by land-based animals like silkworms for the production of their cocoon. There are many successful studies already proving the empirical evidence of biomaterials from land-based silk in biomedical applications. Generally, silk-based hydrogels are mainly involved in the fabrication of different implants for skin, bone, cartilage and vascular-regeneration. The ideal silk fibroin hydrogels for skin, cosmetic and wound healing purposes should exhibit enhanced biological response which is mainly regulated by its tailored mechanical, rheological, viscoelastic properties, effective tissue regeneration ability, controllable swelling, hemostasis and biocompatibility. Accordingly, this review summarizes the rheological and viscoelastic properties of silk-fibroin based composite hydrogels obtained from various raw materials/composites, highlighting the relation of its rheological response to hydrogel biomaterial functions aiming biomedical applications.

Automated summary

Hydrogel-based systems are widely used for 3D-cell culture, and new approaches are being developed to create better polymeric biomaterials for tissue regeneration. Land and marine-based polymers, including silk, have been explored for biomedical applications. Silk-based hydrogels are mainly used for various implants and require specific mechanical, rheological, viscoelastic properties, tissue regeneration ability, and biocompatibility.