Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 120, Issue 1, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2120582120
Keywords
genetic tool; intercellular interactions; membrane-permeable protein; liver zonation; niche
Categories
Funding
- National Key Research amp
- Development Program of China [2019YFA0110403, 31922032, 2018YFA0107900]
- NSF of China [82088101, 31730112, 32050087, JCYJ-SHFY-2021-0]
- Shanghai Pilot Program for Basic Research-Chinese Academy of Science, Shanghai Branch [2018YFA0108100]
- China Postdoctoral Science Foundation
- XPLORER PRIZE
- [2019YFA0802000]
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Understanding cell-cell interactions is crucial for understanding biological processes. In this study, we developed a labeling strategy called Cre-induced intercellular labeling protein (CILP) to label neighboring cells in mammals. By using CILP, we successfully labeled endothelial cells in adult mice and revealed their gene signatures.
Unraveling cell-cell interaction is fundamental to understanding many biological processes. To date, genetic tools for labeling neighboring cells in mammals are not available. Here, we developed a labeling strategy based on the Cre-induced intercellular labeling protein (CILP). Cre-expressing donor cells release a lipid-soluble and membrane-permeable fluorescent protein that is then taken up by recipient cells, enabling fluorescent labeling of neighboring cells. Using CILP, we specifically labeled endothelial cells surrounding a special population of hepatocytes in adult mice and revealed their distinct gene signatures. Our results highlight the potential of CILP as a platform to reveal cell-cell interactions and communications in vivo.
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