4.6 Article

Importance of annexin V N-terminus for 2D crystal formation and quick purification protocol of recombinant annexin V

Journal

PLOS ONE
Volume 17, Issue 12, Pages -

Publisher

PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0278553

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The N-terminal of annexin V has significant influence on 2D crystal formation, as observed using high-speed atomic force microscopy (HS-AFM). Furthermore, a quick purification method is presented to purify recombinant annexin V without any residual affinity tag after protein purification in approximately 3 hours.
Annexin V forms trimeric structures which further assemble into two-dimensional crystal (2D crystal) lattices on negatively charged phospholipid bilayer in a Ca2+-dependent manner. It is also known that annexin V 2D crystals show two types of symmetric patterns with six-fold symmetry (p6) and three-fold symmetry (p3). The p6 lattice also contains additional trimers in the gaps between the p6 axes, which are also referred to as non-p6 trimers because they do not participate in the formation of the p6 lattice. We here show that the annexin V N-terminal has significant influence on 2D crystal formation using high-speed atomic force microscopy (HS-AFM) observations. We also present a quick purification method to purify recombinant annexin V without any residual affinity tag after protein purification in similar to 3h.

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