Arabidopsis MPK3 and MPK6 regulates D-glucose signaling and interacts with G-protein, RGS1

Sugar as a signaling molecule has attracted lots of attention. Even though several kinases have been shown to play a crucial role in the sugar signaling and response to exogenous D-glucose (Glc), the information on the involvement of MAP kinase cascade in sugar signaling has remain largely unexplored. In this report we demonstrate that MAP kinase signaling is essential for sensitivity to higher concentrations of D-Glc in Arabi-dopsis. We found that D-Glc activates MAP kinases, MPK3 and MPK6 in a concentration and time-dependent manner. The mutants of mpk3 and mpk6 display hyposensitivity to 6% D-Glc during seed germination, coty-ledon greening and root growth. Interestingly, the altered sensitivity to increased D-Glc is severely enhanced by addition of 1% Sucrose in the media. Our study also deciphered the role of one of the Glc sensor proteins, RGS1 that interacts and gets phosphorylated at its C-terminal domain by MPK3 and MPK6. Overall our study provides a new insight on the involvement of MAP kinases in association with G-proteins that might regulate sugar signaling and sugar responsive growth and development in Arabidopsis.

Automated summary

This study reveals the essential role of MAP kinase signaling in Arabidopsis sensitivity to higher concentrations of D-Glc. D-Glc activates MAP kinases MPK3 and MPK6 in a concentration and time-dependent manner. Mutants of mpk3 and mpk6 display low sensitivity to D-Glc, and this sensitivity is further enhanced by the addition of sucrose. The study also provides insights into the interaction between the Glc sensor protein RGS1 and MPK3/MPK6.