4.8 Article

A contemporary reassessment of the enhanced transient expression system based on the tombusviral silencing suppressor protein P19

Journal

PLANT JOURNAL
Volume 113, Issue 1, Pages 186-204

Publisher

WILEY
DOI: 10.1111/tpj.16032

Keywords

transient expression; agro-infiltration; RNA silencing; P19 protein; siRNAs; technical advance

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Transient transgenic expression is a useful tool for pharming and protein studies in plants. However, post-transcriptional gene silencing (PTGS) impedes gene expression during 'agro-infiltration'. Co-expression of the tombusvirus-encoded P19 protein can suppress PTGS and enhance transient gene expression. This study investigates the mechanisms and limitations of the P19 system, providing insights for its optimization and application.
Transient transgenic expression accelerates pharming and facilitates protein studies in plants. One embodiment of the approach involves leaf infiltration of Agrobacterium strains whose T-DNA is engineered with the gene(s) of interest. However, gene expression during 'agro-infiltration' is intrinsically and universally impeded by the onset of post-transcriptional gene silencing (PTGS). Nearly 20 years ago, a simple method was developed, whereby co-expression of the tombusvirus-encoded P19 protein suppresses PTGS and thus enhances transient gene expression. Yet, how PTGS is activated and suppressed by P19 during the process has remained unclear to date. Here, we address these intertwined questions in a manner also rationalizing how vastly increased protein yields are achieved using a minimal viral replicon as a transient gene expression vector. We also explore, in side-by-side analyses, why some proteins do not accumulate to the expected high levels in the assay, despite vastly increased mRNA levels. We validate that enhanced co-expression of multiple constructs is achieved within the same transformed cells, and illustrate how the P19 system allows rapid protein purification for optimized downstream in vitro applications. Finally, we assess the suitability of the P19 system for subcellular localization studies - an originally unanticipated, yet increasingly popular application - and uncover shortcomings of this specific implement. In revisiting the P19 system using contemporary knowledge, this study sheds light onto its hitherto poorly understood mechanisms while further illustrating its versatility but also some of its limits.

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