Establishment of an efficient method for kiwifruit in vitro micrografting

Key message The results showed that the best conditions for in vitro grafting of kiwifruit were that the rootstock is not rooted and that both rootstock and scion were free of leaves. The best medium formula suitable for kiwifruit micrografting was 1/2 MS solid medium supplemented with 0.5 mg L-1 GA3, 1.0mg L-1 IBA, 40 g L-1 sucrose, and 7 g L-1 agar, pH 6.0. The perlite: peat soil: vermiculite volume ratio at 1:2:1 was the most suitable stroma formula for transplanting micrografted seedlings. This study was carried out to assess the potential use and applicability of micrografting technique for developing in vitro grafted plantlets. Microshoots of kiwifruit 'Miliang-1' and 'Hongyang' were used as rootstock and scion. Accomplishment of in vitro grafting has been examined by varying numerous factors, the physiological state of the rootstock and scion, the pH value of the medium, the concentration of sucrose, the type of medium and the PGRs. The results showed that the best conditions for in vitro grafting of kiwifruit were that the rootstock is not rooted and that both rootstock and scion were free of leaves. The best medium formula suitable for kiwifruit micrografting was 1/2 MS solid medium supplemented with 0.5 mg L-1 GA(3), 1.0 mg L-1 IBA, 40 g L-1 sucrose and 7 g L-1 agar, pH 6.0. The perlite: peat soil: vermiculite volume ratio at 1:2:1 was the most suitable stroma formula for transplanting micrografted seedlings.

Automated summary

The best conditions for in vitro grafting of kiwifruit were identified, including the usage of rootstock without roots and both rootstock and scion free of leaves. The most suitable medium formula for kiwifruit micrografting was determined to be 1/2 MS solid medium supplemented with 0.5 mg L-1 GA3, 1.0 mg L-1 IBA, 40 g L-1 sucrose, and 7 g L-1 agar, pH 6.0. Additionally, the perlite: peat soil: vermiculite volume ratio at 1:2:1 was found to be the most suitable stroma formula for transplanting micrografted seedlings.