Multi-gene phylogeny reveals a new genus and species of Hapalidiales (Rhodophyta) from Antarctica: Thalassolithon adeliense gen. & sp. nov.

Non-geniculate coralline algal specimens were collected in 2013 during the XXVIII Italian Expedition to Antarctica in Adelie Cove (Terra Nova Bay; Ross Sea) and deposited in the collections of the Italian National Antarctic Museum (MNAIT, Section of Genoa). Specimens were characterized through a polyphasic approach combining DNA sequence data obtained for four genes (psbA, rbcL, 18S rDNA and cox1) with morpho-anatomical observations. DNA sequences revealed that all specimens belonged to the same species. Phylogenetic reconstructions unambiguously recovered this alga as a member of the order Hapalidiales, but without any close relationship to a genus of this order currently recognized on a molecular phylogenetic basis. Instead, it formed a well-supported lineage with specimens named 'Hapalidiales sp. ZH-Twist-2019', collected in New Zealand, for which no formal assignment at genus level has been proposed. Species delimitation methods (ABGD, PTP, GMYC) applied to the psbA dataset indicated that the Adelie Cove coralline alga is a distinct species from all other known hapalidialean species for which such sequences are available. A new genus, Thalassolithon gen. nov., is proposed for T. adeliense sp. nov.

Automated summary

During the XXVIII Italian Expedition to Antarctica in 2013, non-geniculate coralline algal specimens were collected in Adelie Cove and deposited in the collections of the Italian National Antarctic Museum. Through a combination of DNA sequence data and morpho-anatomical observations, the specimens were characterized and identified as a new species, which belongs to the order Hapalidiales but is distinct from any known genus in this order. A new genus, Thalassolithon gen. nov., is proposed for this newly discovered species, named T. adeliense sp. nov.