Field evaluation of glutathione and glutamic acid as host marking pheromones for control of Tephritid fruit flies in a mango orchard in Kenya

BACKGROUND Tephritid fruit flies are a major constraint to the global production of horticultural crops. In Africa, an array of native and alien invasive fruit fly species represents a key challenge to the horticultural industry. In an effort to develop a safer management tool for these pests, we previously identified glutathione (GSH) and glutamic acid (GA), as the host marking pheromones (HMPs) of Ceratitis cosyra and Ceratitis rosa, respectively. Here, we report on the effectiveness of these compounds (GSH and GA) in reducing natural fruit fly population infestations in mango orchards. Mango trees at two different agroecological zones in Kenya were sprayed with HMPs, and the fruits sampled periodically and assessed for fruit fly emergence. Fruit fly emergence data were compared to two controls, the positive control using spot spraying of food bait (SS), and the negative control using water (W). RESULTS The two HMPs and SS substantially decreased fruit fly emergence from the sampled mangoes. GSH and GA treated mangoes showed reduced C. cosyra and C. rosa infestation by similar to 70-75% relative to control (W), and with variation noted in the pheromone applied and the recovered fruit fly species. The adult emergence in pheromone-treated plants compared favorably with the positive control SS. However, the HMPs had little impact on reducing Bactrocera dorsalis infestation of mangoes. CONCLUSION The decrease in fruit fly emergence in sampled mango fruits from HMP treated trees corroborate previous laboratory results and support the prospect of using HMPs in the management of African fruit fly species. (c) 2022 Society of Chemical Industry.

Automated summary

The study found that glutathione (GSH) and glutamic acid (GA) can effectively reduce the offspring population of African fruit flies (Ceratitis cosyra and Ceratitis rosa) in Kenyan mango orchards, but have little impact on Bactrocera dorsalis. This research provides a potential method for managing African fruit fly species using host marking pheromones (HMPs).