Overexpression of PRKCH promotes tumorigenesis in patients with glioma and influences glioma stem cell properties

Background: PRKCH is a member of the PKC family with the potential to regulate cell proliferation and differ-entiation. Glioma is the most common primary tumor of the central nervous system, with a high recurrence rate and poor prognosis. Recent studies have demonstrated that PRKCH can promote the proliferation of glioma cells. The purpose of this study was to investigate the promoting effect and possible mechanism of PRKCH on glioma development.Methods: Tumor tissue and paracancerous tissue were collected from 160 glioma patients treated at the General Hospital of Northern Theater Command. The expression level of PRKCH was detected by immunohistochemistry and immunoblotting. Univariate/multivariate analysis and log-rank analysis, as well as prognosis and survival analysis, were performed using SPSS26 software. The PRKCH overexpression model was constructed in vitro to study the effect of PRKCH expression on the characteristics of glioma stem cells.Results: The expression of PRKCH in glioma tissues was higher than that in adjacent tissues. PRKCH expression level is an independent prognostic factor in glioma patients, promoting poor prognosis and shorter survival in glioma patients. Furthermore, overexpression of PRKCH in glioma stem cells significantly increased stem cell properties and enhanced cell viability. Downregulation of PRKCH significantly inhibited the progression of glioma stem cells.Conclusion: PRKCH promotes the development of gliomas and may be a therapeutic target for gliomas.

Automated summary

This study found that PRKCH expression in glioma tissues was higher than in adjacent tissues and it is an independent prognostic factor, promoting poor prognosis and shorter survival in glioma patients. In addition, overexpression of PRKCH significantly increased stem cell properties and enhanced cell viability in glioma stem cells.