Antimicrobial evaluation of bismuth subsalicylate nanoparticles synthesized by laser ablation against clinical oral microorganisms

Nowadays, nanotechnology can be useful to generate novel therapeutic compounds with antimicrobial properties for the treatment of different oral infections, such as periodontal diseases. In this work, the antibacterial activity of bismuth subsalicylate nanoparticles (BSS-NPs) synthesized by laser ablation of solids in liquids (LASL) was evaluated against microorganisms taken from subgingival biofilm samples of patients with periodontal disease. The physicochemical characterization of the BSS-NPs was performed using different techniques, such as Ultra-violet-Visible Spectroscopy (UV-Vis); X-Ray Diffraction (XRD); Transmission Electron Microscopy (TEM) and Fourier Transform Infrared Spectroscopy (FT-IR). The evaluation of the antibacterial activity of the BSS-NPs (16.6 mu g/mL) against the oral clinical samples, was performed using the colony-forming unit assay. In addi-tion, 16S rRNA sequencing was used to identify sensitive and resistant bacterial genera to the BSS-NPs. The characterization results showed that the synthesized BSS-NPs by laser ablation of solids in liquids maintained the triclinic crystallographic structure, and the vibrational modes of the functional groups of bismuth subsalicylate. Moreover, BSS-NPs presented a quasi-spherical shape, an average size of 4 nm, and water -suspended stability for up to 170 days. Regarding the antimicrobial activity, more than 30 % of the total cultivable bacteria from the oral biofilm samples were inhibited when exposed to BSS-NPs. In addition, sensitive microorganisms to the BSS-NPs such as Aggregatibacter, Streptococcus, Veillonella, Eikenella, Peptostreptococcus, Eubacterium, Prevotella, Selenomonas, Haemophilus, Filifactor, and others, were identified by bioinformatic analysis of 16S rRNA sequencing. Therefore, BSS-NPs have the potential for being used as a new alternative antimicrobial in the treatment of periodontal diseases, reducing the indiscriminate use of antibiotics and preventing the in-crease of antibiotic resistance.

Automated summary

Nowadays, nanotechnology is being used to develop novel therapeutic compounds for the treatment of oral infections. In this study, bismuth subsalicylate nanoparticles (BSS-NPs) synthesized through laser ablation of solids in liquids (LASL) showed antibacterial activity against microorganisms from periodontal disease patients. The physicochemical characterization of the BSS-NPs revealed their triclinic crystallographic structure, functional group vibrational modes, quasi-spherical shape, and size. The BSS-NPs exhibited water-suspended stability and inhibited over 30% of cultivable bacteria from oral biofilm samples, including Aggregatibacter, Streptococcus, Veillonella, Eikenella, Peptostreptococcus, Eubacterium, Prevotella, Selenomonas, Haemophilus, and Filifactor, as determined by 16S rRNA sequencing.